The unsubsidised DL06 route will connect Sai Gon Bus Station and Binh Trung, covering about 27 km in a 110-minute journey.
Operated by World Travel Trading JSC, the route will use 60-seat open-top double-decker buses, allowing passengers to view the city from an elevated vantage point while passing major landmarks and modern urban areas.
The service will operate daily from 8 a.m. to 7 p.m., with five trips a day from July 4 to Sept. 30, increasing to 10 daily trips in October and 15 from November onwards.
The new service will bring the number of open-top tourist bus routes in the city to six, most of which operate around the city centre.
City officials said the new route would diversify tourism products, improve visitor experiences and promote Ho Chi Minh City as a vibrant and visitor-friendly destination.